LOCUS       sequence-93625-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-nEF Con/Fon hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(189..222)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(273..306)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
       61 gcactgcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagctgtgg
      121 gacgacatga cttaaccagt taaccatgtt ctctgaacat gagaagggct ggccactctc
      181 cagtcgacat aacttcgtat aggatacttt atacgaagtt atgcagaatg gtagctggat
      241 tgtagctgct attagcaata tgaaacctct taataacttc gtatagtata ccttatacga
      301 agttatacta gttgtgcccc cccttttttt tataaattgt attaatgtta tatacatatc
      361 tcctgtatgt gacccatgtg cttatgactc tatttctcat gtgtttaggc ctgagcaacg
      421 actacagcag gagaaccatg ggactccttg tctcagacat cgggactatc gtgtgggggg
      481 ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt ggattgtgct
      541 atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat catactgtgc
      601 caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc gtgagctggg
      661 gtatgttccc aattctcttc attttggggc
//
