LOCUS       sequence-93627-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses CKK domain of CAMSAP3 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pET-28a+GFP-CAMSAP3 CKK
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             586..603
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(656..674)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      670..717
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggatccgaaa atctgtattt tcaggggggt ggaggtggta cacccaataa ccttggtgtg
       61 aagaggccca cctctcgggc cccttctcca tcaggcctca tgtctccaag ccgcctgcct
      121 ggcagtcgag aacgtgactg ggagaatgga agcaatgcat cctccccagc atcagtgcct
      181 gagtacacag gtccccggct atacaaggaa cccagcgcca agtctaacaa gtttatcatc
      241 cacaatgcct tgtcacactg ctgcctggca ggcaaggtga atgagcccca gaagaacaga
      301 attctagagg aaatcgagaa aagcaaggcc aaccacttcc tgattctctt tcgggactca
      361 agctgccagt tccgggccct ctacactctg tctggggaga cagaggagct atcgaggctg
      421 gcaggctatg ggcctcggac cgtcactcct gccatggtcg aaggcatcta taagtacaac
      481 tcggatcgca aacggttcac ccagatccct gctaaaacca tgtctatgag tgtagacgcc
      541 ttcactatcc agggacacct ttggcaaagc aagtaggcac tcgagcacca ccaccaccac
      601 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      661 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      721 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gc
//
