LOCUS       sequence-93639-e        3209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Folding reporter with ampicillin resistance as a readout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-EcoBam
FEATURES             Location/Qualifiers
     source          1..3209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             159..947
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(305..324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1251..1910)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1825..1844
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1911..2013)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2233..2821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2722..2741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2975..2992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3109..3130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3145..3150,3151..3168,3169..3175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggatctcgac atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
       61 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgctaacgc cgcgacgtgc
      121 gactgcgcat atggaattct ccagacgacg gatcccaccc agaaacgctg gtgaaagtaa
      181 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      241 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      301 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
      361 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      421 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      481 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      541 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      601 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
      661 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      721 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      781 aatctggagc aggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      841 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      901 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaagat ttttccaatg
      961 atgagcactt tttgatcaga aaaaaaggat catatcgtca attattacct ccacggggag
     1021 agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
     1081 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
     1141 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
     1201 caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     1261 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1321 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1381 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1441 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     1501 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1561 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1621 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1681 attgccatac gtaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1741 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1801 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1861 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1921 ttagctcctg aaaatctcga caactcaaaa aatacgcccg gtagtgatct tatttcatta
     1981 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     2041 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     2101 gtcacaggta tttattcggt cgaaaaggat ctaggtgaag atcctttttg ataatctcat
     2161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3001 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3061 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     3121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     3181 aattgtgagc gggataacaa tttcacaca
//
