LOCUS       sequence-9364-ex        9692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Stadtfeld M, Maherali N, Breault DT, Hochedlinger K
  TITLE     Defining molecular cornerstones during fibroblast to iPS cell 
            reprogramming in mouse.
  JOURNAL   Cell Stem Cell. 2008 Mar 6. 2(3):230-40.
  PUBMED    18371448
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Mar 6. 2(3):230-40."
COMMENT     Sequence Label: pLV-tetO-Oct4
FEATURES             Location/Qualifiers
     source          1..9692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2610..2628,2629..2651,2652..2670,2671..2693,2694..2712,
                     2713..2735,2736..2754,2755..2777,2778..2796,2797..2819,
                     2820..2838,2839..2861,2862..2880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2950
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2947..2971
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4384..4972
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4437..4457)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4855..4866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4975..4991)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4976..4992)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5497..5677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5703..5720)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5709..5933
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5979..6407
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6066..6085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6276..6297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6416..6436)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6421..6750
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6601..6736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6663..6682
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6798..6845
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6864..7238
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7368..7489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7405..7424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7459..7478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7538..7554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7538..7554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7551..7573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7562..7578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7586..7592,7593..7610,7611..7616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7631..7652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7769..7786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7940..8528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8020..8039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8699..9490,9491..9559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9322..9341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9560..9664)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
     2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg ccctttgagc
     3061 cgtctttcca ccaggccccc ggctcggggt gcccaccttc cccatggctg gacacctggc
     3121 ttcagacttc gccttctcac ccccaccagg tgggggtgat gggtcagcag ggctggagcc
     3181 gggctgggtg gatcctcgaa cctggctaag cttccaaggg cctccaggtg ggcctggaat
     3241 cggaccaggc tcagaggtat tggggatctc cccatgtccg cccgcatacg agttctgcgg
     3301 agggatggca tactgtggac ctcaggttgg actgggccta gtcccccaag ttggcgtgga
     3361 gactttgcag cctgagggcc aggcaggagc acgagtggaa agcaactcag agggaacctc
     3421 ctctgagccc tgtgccgacc gccccaatgc cgtgaagttg gagaaggtgg aaccaactcc
     3481 cgaggagtcc caggacatga aagccctgca gaaggagcta gaacagtttg ccaagctgct
     3541 gaagcagaag aggatcacct tggggtacac ccaggccgac gtggggctca ccctgggcgt
     3601 tctctttgga aaggtgttca gccagaccac catctgtcgc ttcgaggcct tgcagctcag
     3661 ccttaagaac atgtgtaagc tgcggcccct gctggagaag tgggtggagg aagccgacaa
     3721 caatgagaac cttcaggaga tatgcaaatc ggagaccctg gtgcaggccc ggaagagaaa
     3781 gcgaactagc attgagaacc gtgtgaggtg gagtctggag accatgtttc tgaagtgccc
     3841 gaagccctcc ctacagcaga tcactcacat cgccaatcag cttgggctag agaaggatgt
     3901 ggttcgagta tggttctgta accggcgcca gaagggcaaa agatcaagta ttgagtattc
     3961 ccaacgagaa gagtatgagg ctacagggac acctttccca gggggggctg tatcctttcc
     4021 tctgccccca ggtccccact ttggcacccc aggctatgga agcccccact tcaccacact
     4081 ctactcagtc ccttttcctg agggcgaggc ctttccctct gttcccgtca ctgctctggg
     4141 ctctcccatg cattcaaact gaggcaccag ccctccctgg ggatgctgtg agccaaggca
     4201 agggaggtag acaagagaac ctggagcttt ggggttaaat tcttttactg aggagggatt
     4261 aaaagcacaa caggggtggg gggtgggatg gggaaagaag ctcagtgatg ctgttgatca
     4321 ggagcctggc ctgtctgtca ctcattttgt tctaagggcg aattcgatat caagcttatc
     4381 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4441 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4501 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4561 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4621 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4681 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4741 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4801 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4861 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4921 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     4981 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     5041 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     5101 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5161 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     5221 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5281 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     5341 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     5401 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     5461 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     5521 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5581 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5641 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     5701 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5761 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5821 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5881 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5941 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6001 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6061 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6121 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6181 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6241 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6301 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6361 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6421 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6481 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6541 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6661 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6721 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6781 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     6841 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     6901 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     6961 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     7021 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     7081 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     7141 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     7201 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     7261 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7321 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7381 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7441 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7501 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7561 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7621 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7681 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7741 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8341 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9301 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9661 cgcgcacatt tccccgaaaa gtgccacctg ac
//
