LOCUS       sequence-93643-e        4012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSALECT with SupH insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-SupH
FEATURES             Location/Qualifiers
     source          1..4012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             962..1750
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(1108..1127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2054..2713)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2628..2647
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2714..2816)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      3036..3624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3525..3544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3778..3795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3912..3933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3948..3953,3954..3971,3972..3978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggatctcgac atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
       61 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgctaacgc cgcgacgtgc
      121 gactgcgcat atggaattca tgtctgtaaa agttatagtc accgatatgg acggaacttt
      181 cttgaacgac gccaaaacgt acaatcagcc tcgtttcatg gcacaatacc aagagttgaa
      241 gaaacgtggc attaaattcg tcgtggcgag cgggaaccaa tactatcaac ttatcagttt
      301 tttcccggag ttgaaggacg aaatctcctt tgtagctgaa aatggtgcgt tagtttatga
      361 gcatggtaag caactatttc acggagagct gacaaggcac gaaagccgta tagtcatcgg
      421 ggaattacta aaggataaac aacttaattt cgttgcatgt ggactgcaat ctgcatatgt
      481 ctcggaaaat gcgcccgaag cgtttgtcgc cttgatggca aaacattatc ataggttgaa
      541 acctgtcaag gattaccagg aaattgacga tgtccttttt aaattctcct tgaacttgcc
      601 tgatgagcaa atcccactgg tcatcgacaa attacacgtc gcgttggacg gtataatgaa
      661 gcctgttaca agtgggtttg ggttcattga tttaatcata ccaggtcttc acaaagcgaa
      721 cggcatttct aggttactaa aaagatggga tttgtctcca caaaacgttg tcgcaatcgg
      781 cgattcaggc aacgacgcag aaatgttaaa gatggcaagg tatagcttcg caatgggtaa
      841 tgccgcagaa aacataaaac aaatagcaag atatgcaacg gacgataaca atcatgaggg
      901 ggcgctaaat gtcatccaag cagttttgga taacacttcg cccttcaact cgggatccca
      961 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1021 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1081 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     1141 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1201 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1261 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1321 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1381 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     1441 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1501 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1561 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1621 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1681 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1741 gcattggtaa gatttttcca atgatgagca ctttttgatc agaaaaaaag gatcatatcg
     1801 tcaattatta cctccacggg gagagcctga gcaaactggc ctcaggcatt tgagaagcac
     1861 acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct
     1921 atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat
     1981 tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac
     2041 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2101 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2161 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     2221 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     2281 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2341 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     2401 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2461 atatcaccag ctcaccgtct ttcattgcca tacgtaattc cggatgagca ttcatcaggc
     2521 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2581 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2641 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2701 tttttttctc cattttagct tccttagctc ctgaaaatct cgacaactca aaaaatacgc
     2761 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     2821 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     2881 tttattctgc gaagtgatct tccgtcacag gtatttattc ggtcgaaaag gatctaggtg
     2941 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3001 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3061 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3901 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     3961 cttccggctc gtatgttgtg tggaattgtg agcgggataa caatttcaca ca
//
