LOCUS       sequence-93644-e        3814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSALECT with PRAI insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-PRAI
FEATURES             Location/Qualifiers
     source          1..3814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             764..1552
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     complement(910..929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1856..2515)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2430..2449
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2516..2618)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2838..3426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3327..3346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3580..3597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3714..3735
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3750..3755,3756..3773,3774..3780)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggatctcgac atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
       61 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgctaacgc cgcgacgtgc
      121 gactgcgcat atggaattca tggttagagt taaaatctgc ggtattacta accttgagga
      181 cgcacttttt tccgtagaat ctggggctga tgctgtcgga ttcgtttttt atcctaaaag
      241 taagagatat atcagtcccg aagatgccag aaggatctcc gtggaactgc cgccgtttgt
      301 cttcagggtg ggtgtcttcg tcaatgagga gcctgagaaa atcctggatg tggcctcgta
      361 tgtgcagttg aatgctgtcc agctacatgg agaagaacct atagagcttt gtcgtaaaat
      421 tgccgaaagg atcttggtga ttaaagctgt tggtgtctct aacgaaagag acatggagag
      481 ggcgctaaac taccgtgaat ttcccatttt acttgacact aaaactcctg aatatggtgg
      541 atctggtaaa acatttgatt ggtcactgat cctgccttac agagaccgtt tcagatatct
      601 agtgttaagc ggcggcctaa atccagagaa tgtgaggagt gccatagatg ttgttagacc
      661 tttcgcagtg gatgtctctt ccggggtcga agccttccca ggcaaaaagg accacgattc
      721 cattaagatg ttcatcaaga acgctaaagg tttgggatcc cacccagaaa cgctggtgaa
      781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      901 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
      961 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1021 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1081 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1141 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1201 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     1261 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1321 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1381 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1441 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1501 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aagatttttc
     1561 caatgatgag cactttttga tcagaaaaaa aggatcatat cgtcaattat tacctccacg
     1621 gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg
     1681 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     1741 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     1801 ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
     1861 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1921 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1981 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2041 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     2101 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2161 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     2221 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2281 ctttcattgc catacgtaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2341 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2401 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2461 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2521 cttccttagc tcctgaaaat ctcgacaact caaaaaatac gcccggtagt gatcttattt
     2581 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     2641 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     2701 cttccgtcac aggtatttat tcggtcgaaa aggatctagg tgaagatcct ttttgataat
     2761 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3481 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3541 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3601 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3661 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     3721 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     3781 tgtggaattg tgagcgggat aacaatttca caca
//
