LOCUS       sequence-93657-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses CKK domain of CAMSAP2 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pET-28a+GFP-CAMSAP2 CKK
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             467..484
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(537..555)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      551..598
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgtatttt caggggggtg gaggtggtac atctggaaca gaatatacag gaccaaagct
       61 ctacaaagaa cccagtgcaa aatccaataa gcacataata caaaatgctt tagctcattg
      121 ctgtttggct ggaaaagtaa atgaaggtca gaagaaaaaa atactggagg aaatggagaa
      181 atcagatgcc aacaacttct taatcttgtt ccgggattca ggatgccagt tcagatcttt
      241 atacacttat tgcccagaaa ctgaagaaat caataaactg actgggatag gccctaaatc
      301 tatcactaaa aaaatgattg aaggacttta caaatataat tctgacagga aacagtttag
      361 ccacataccc gctaaaactt tatctgccag tgttgatgca attaccattc atagccattt
      421 atggcagacc aaaagaccag taacacccaa aaaataggca ctcgagcacc accaccacca
      481 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
      541 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      601 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac
//
