LOCUS       sequence-93697-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mVenus-fusion protein of VfAU1-LOV domain (Vaucheria 
            frigida Aureochrome 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: mV-VfAU1-LOV_226
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(505..756)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(547..568)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     896..915
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgagctcgga tccgctttgt tagcagccgg atcaagcttt tatccgggct ttctgcgcag
       61 catgttactg gtgcgcacac ctttgtactc aatgttctgc tcgttgacca gcagcttggc
      121 ataatcttcg ctcacctttg actgcactcc gacgtagttg acaatattgc ccttagaatc
      181 tctgagtcca gccacgaaga agagattcca gaaggttgtg ccatcctgtc tataattcag
      241 cagacagaca ctggtatcaa cgcctttggt gatggcattc ctgatcttat ccacagctct
      301 tgggtctgtt tctggccctt gcagaaacct gcagttcctg cccaggatct ggtcgagaga
      361 atagcctgtc agtgtcagaa accctctact ggcgtagacg atagggttgt ctgggaggga
      421 ggcgtctgta atgacaaaat tctgttgtgc catttgcaga gccttcacga gactgtagtc
      481 aggaccggat gatcctgaac tgcccttgta cagctcgtcc atgccgagag tgatcccggc
      541 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      601 tttggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      661 ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt tgtggcggat
      721 cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct
      781 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
      841 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
      901 gtagttgccg tcgtccttga
//
