LOCUS       sequence-93698-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mVenus-fusion protein of CrPH-LOV1 domain (Chlamydomonas 
            reinhardtii Phototropin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: mV-CrPH-LOV1_227
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(423..674)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(465..486)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     814..833
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagctcggat ccgctttgtt agcagccgga tcaagctttt atccgggggc cagggctttc
       61 ccttcagtct tggaggtaac atccacctgc acgccgacaa acttggacac gcggccatca
      121 ggggttttaa ttggggtgac tgtcagcagg ttccagaagg gagtgccgtc cttccgatag
      181 ttcaggaggc gcacactaca agcctcaccc ttcttgatgg cgtcgcgaat tttctgcact
      241 tccttggggt cagttccctc accctgcaga aacctacagt tgtgacccag cacttcgtca
      301 ggtccatatc cggtcattgc gtagaagccc tcacttgcat agaccagtgg gcaatcaggg
      361 agtgttgcat cagccaccac aaatgtatgt ctgagtcctg caccggatga tcctgaactg
      421 cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      481 accatgtgat cgcgcttctc gttggggtct ttgctcagtt tggactggta gctcaggtag
      541 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      601 gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg
      661 ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      721 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      781 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      841 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaa
//
