LOCUS       sequence-93699-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucN-hDib1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-NO-Dib1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(130..148)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat
      121 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
      181 attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg
      241 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
      301 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
      361 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac
      421 atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
      481 caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag
      541 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
      601 gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat gaactcctct
      661 ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc
      721 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
      781 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
      841 tttcgagtcg tcttaatgta taga
//
