LOCUS       sequence-93700-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mVenus-fusion protein of AtPH1-LOV2 domain (Arabidopsis 
            thaliana Phototropin 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: mV-AtPH1-LOV2_229
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(483..734)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(525..546)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     874..893
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagctcggat ccgctttgtt agcagccgga tcaagctttt atccgggccg cacgggctca
       61 acgtgcttgc tgccatccag ctgcaccccg atgaaatatt ggacctcacc cttctggtcc
      121 cgcataggct gcaggtggaa aatgttccag aacttcttgc cgcttttagt gtagtttatc
      181 agttgcacgg taacctcggt ttgattatcg atagcattgc gaatcttctt cacggtggtc
      241 aggtctgtct cgggtccctg caggaatctg caatttctac ccaggatctc ttcccggcta
      301 tactctgtca gctccaggaa actgtctgaa gcgaagatta tgggattatc ggggagccta
      361 gggtcagtaa tcacaaagtt cttttctatt ctttcgagtg ttgttgcgag atcgattccc
      421 tttctcattt ccttctgtct gaccttatca tcaacgcttt caccggatga tcctgaactg
      481 cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      541 accatgtgat cgcgcttctc gttggggtct ttgctcagtt tggactggta gctcaggtag
      601 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      661 gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg
      721 ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      781 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      841 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      901 aagatggtgc gctcctggac gtagccttcg g
//
