LOCUS       sequence-93701-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express hDib1-LucN fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-ON-hDib1
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             70..93
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(554..580)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
     primer_bind     complement(652..670)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttatgg actacaagga cgacgatgac aaggaactag ctctagttga attcatgtcg
      121 tacatgctcc cgcacctgca caacggctgg caggtggacc aggccatcct ctcggaggag
      181 gaccgcgtgg tcgtcatccg cttcggccac gactgggatc ctacgtgcat gaagatggac
      241 gaggtcctgt acagcatcgc cgagaaggtt aaaaattttg cagttattta tcttgtggat
      301 attacagaag tgcctgactt caacaaaatg tatgagttat acgatccatg tactgtcatg
      361 tttttcttca ggaacaagca catcatgatt gacttgggga ctggcaacaa caacaagatt
      421 aactgggcca tggaggacaa gcaggagatg gtggacatca tcgagacggt gtaccgcggg
      481 gcccgcaaag gccgcggcct ggtggtgtcc cccaaggact actccaccaa gtaccgctac
      541 ctcgagggta ccggtggtgg tggtggtagt ggtggtggtg gatccatgga agacgccaaa
      601 aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc tggagagcaa
      661 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      721 catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa
      781 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tg
//
