LOCUS       sequence-93702-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mVenus-fusion protein of NcVV-LOV domain (Neurospora 
            crassa Vivid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: mV-NcVV-LOV_231
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(520..771)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(562..583)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     911..930
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgagctcgga tccgctttgt tagcagccgg atcaagcttt tatccgggtt cggtttcgca
       61 ctgaaaaccc atgctgtacc ggtactcccc ggtttcgtcc cgcactggaa tcattgtgag
      121 aaagttcacg aaccgctggc cgttcttctt aaagttcacc acttccacct gaacttctgc
      181 gtttctgtcg atggctttcc tcatggtgtt aatagtattg ctatccacat atttgcgagt
      241 actcttaggt ttcaccatac catcgggact ctggaggaac ctacagttcc tccccagcac
      301 ctcggcgtta gaataccctg tcatgtacag aaaggcttct gaagcgtaaa ctataggtgt
      361 atccttctgc ttcaggtcgc acagtatcag tgcacatgaa gtatccacgg gtcccagctc
      421 gacctgggga ttgggcctgt tcatgatctg gatcagccag cccataatat cgtacccgcc
      481 tggggcgtag agagtgtgcc cggatgatcc tgaactgccc ttgtacagct cgtccatgcc
      541 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      601 ggggtctttg ctcagtttgg actggtagct caggtagtgg ttgtcgggca gcagcacggg
      661 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc
      721 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
      781 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      841 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
      901 ctcggcgcgg gtcttgtagt tgccgtcgtc cttg
//
