LOCUS       sequence-93704-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mVenus-fusion protein of NcWC1-LOV domain (Neurospora 
            crassa White Collar-1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Jul 1. pii: e201387695."
COMMENT     Sequence Label: mV-NcWC1-LOV_368
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(586..837)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(628..649)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgagctcgga tccgctttgt tagcagccgg atcaagcttt tatccgggac tgtgggtata
       61 gttcacttgc attggtccat tgccctcctg acctatgata gcgtcgggac attccacgag
      121 atcgatctga aacccgataa agtaccttat ctcttcagta tcccaaggga ttggtatcat
      181 ggtcaggagg ttgaggaagg gtttcccacc ttttctgtag ttaatgaggg attgttgtat
      241 ctcctggccc tcagcaatag ttttcttcag ggtatacact gcgttgtttt ccacaaattc
      301 gcgtttggtc ccagcctcaa catttccgtc gggagcctgc agaaatctac agtttcttcc
      361 cacgatttcg tgccgagaat atccggtgag attctggaaa ttgtcgctga cgtagatgat
      421 ggggcaatcg ttcagggtca cgtcgcaaac cacgaaggcg cagctcatat ccactgctcc
      481 gagcttcagc tttggatctt tgcgacttgc cacgtaccac agagctctca gcatgtcgaa
      541 tccgctctta ctatagatgc tcttaccgga tgatcctgaa ctgcccttgt acagctcgtc
      601 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      661 ctcgttgggg tctttgctca gtttggactg gtagctcagg tagtggttgt cgggcagcag
      721 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgccgcc
      781 gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc
      841 ggtgatatag acgttgtggc tgttgtagtt gtactccagc t
//
