LOCUS       sequence-93710-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucN alone in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pcDNA3-FLAG-Luc-N
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(203..221)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gcgagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttatggacta caaggacgac gatgacaagg aactagctct
      121 agttgaattc ggatccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
      181 tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
      241 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga
      301 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
      361 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
      421 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
      481 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa
      541 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa
      601 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
      661 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat
      721 aatgaactcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc
      781 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
      841 tgcga
//
