LOCUS       sequence-93712-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucC alone in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pcDNA3-FLAG-Luc-C
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(639..657)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(640..657)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(677..694)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    683..794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttatggact acaaggacga cgatgacaag gaactagctc
      121 tagttgaatt cggatccgga cctatgatta tgtccggtta tgtaaacaat ccggaagcga
      181 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
      241 aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc
      301 aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg
      361 gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
      421 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
      481 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
      541 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt
      601 ccaaattgta actcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
      661 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      721 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      781 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttct
//
