LOCUS       sequence-93713-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in Escherichia coli T7-Notexin with the amino acid 
            substitution D49K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Simonato M, Morbiato L, Zorzi V, Caccin P, Fernandez J, Massimino 
            ML, Polverino de Laureto P, Tonello F
  TITLE     Production in Escherichia coli, folding, purification and 
            characterization of notexin with wild type sequence and with 
            N-terminal and catalytic site mutations.
  JOURNAL   Toxicon. 2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 
            10.1016/j.toxicon.2014.06.009.
  PUBMED    24951874
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicon. 
            2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 
            10.1016/j.toxicon.2014.06.009."
COMMENT     Sequence Label: pET-20b-T7-Ntx-K49
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..67
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             474..491
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(544..562)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      558..605
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(729..748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatggctag catgactgga ggacaacaaa tgggtatcga
       61 aggccgcaat ctcgttcaat tttcttattt aattcaatgt gcaaatcatg gaaaacgtcc
      121 cacttggcat tatatggact atggttgtta ctgtggcgct ggaggatcag gtaccccggt
      181 tgacgaactg gaccgttgct gcaaaatcca caaagactgc tacgacgaag ctggtaaaaa
      241 aggttgcttc ccgaaaatgt ctgcttacga ctactactgc ggtgaaaacg gtccgtactg
      301 ccgtaacatc aaaaaaaaat gcctgcgttt cgtttgcgac tgcgacgttg aagctgcttt
      361 ctgcttcgct aaagctccgt acaacaacgc taactggaac atcgacacca aaaaacgttg
      421 ccagtaggga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc
      481 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      541 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      601 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      781 tcaagctcta aatcgggggc tccctttagg gttccgatt
//
