LOCUS       sequence-93714-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express FLAG-tagged Luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pcDNA3-FLAG-Luciferase
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(204..222)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttatggact acaaggacga cgatgacaag gaactagctc
      121 tagttgaatt cggatccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct
      181 atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc
      241 tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc acgtacgcgg
      301 aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa
      361 atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg
      421 cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc
      481 tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa
      541 aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc atggattcta
      601 aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg
      661 gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca attgcactga
      721 taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg catagaactg
      781 cctgcgtcag attctcgcat gccagagatc cta
//
