LOCUS       sequence-93717-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in Escherichia coli T7-Notexin with the amino acid 
            substitution D49K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Simonato M, Morbiato L, Zorzi V, Caccin P, Fernandez J, Massimino 
            ML, Polverino de Laureto P, Tonello F
  TITLE     Production in Escherichia coli, folding, purification and 
            characterization of notexin with wild type sequence and with 
            N-terminal and catalytic site mutations.
  JOURNAL   Toxicon. 2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 
            10.1016/j.toxicon.2014.06.009.
  PUBMED    24951874
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicon. 
            2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 
            10.1016/j.toxicon.2014.06.009."
COMMENT     Sequence Label: pET-20b-T7-Ntx-K49
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(440..451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     RBS             492..514
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(545..564)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(546..564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccc tactggcaac gttttttggt gtcgatgttc cagttagcgt
      121 tgttgtacgg agctttagcg aagcagaaag cagcttcaac gtcgcagtcg caaacgaaac
      181 gcaggcattt ttttttgatg ttacggcagt acggaccgtt ttcaccgcag tagtagtcgt
      241 aagcagacat tttcgggaag caaccttttt taccagcttc gtcgtagcag tctttgtgga
      301 ttttgcagca acggtccagt tcgtcaaccg gggtacctga tcctccagcg ccacagtaac
      361 aaccatagtc catataatgc caagtgggac gttttccatg atttgcacat tgaattaaat
      421 aagaaaattg aacgagattg cggccttcga tacccatttg ttgtcctcca gtcatgctag
      481 ccatatgtat atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg
      541 gtctccctat agtgagtcgt attaatttcg cgggatcgag atctcgggca gcgttgggtc
      601 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
      661 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
      721 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
      781 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
      841 atgctgctgg ctacc
//
