LOCUS       sequence-93718-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucN-hDib1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-NO-Dib1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(482..505)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      505..514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             520..546
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
ORIGIN
        1 tagggccctc tagatgcatg ctcgatcact cgaggtagcg gtacttggtg gagtagtcct
       61 tgggggacac caccaggccg cggcctttgc gggccccgcg gtacaccgtc tcgatgatgt
      121 ccaccatctc ctgcttgtcc tccatggccc agttaatctt gttgttgttg ccagtcccca
      181 agtcaatcat gatgtgcttg ttcctgaaga aaaacatgac agtacatgga tcgtataact
      241 catacatttt gttgaagtca ggcacttctg taatatccac aagataaata actgcaaaat
      301 ttttaacctt ctcggcgatg ctgtacagga cctcgtccat cttcatgcac gtaggatccc
      361 agtcgtggcc gaagcggatg acgaccacgc ggtcctcctc cgagaggatg gcctggtcca
      421 cctgccagcc gttgtgcagg tgcgggagca tgtacgacat gaattcaact agagctagtt
      481 ccttgtcatc gtcgtccttg tagtccatgg tggcggatcc accaccacca ctaccaccac
      541 caccaccggt acccataatc ataggtcctc tgacacataa ttcgcctctc tgattaacgc
      601 ccagcgtttt cccggtatcc agatccacaa ccttcgcttc aaaaaatgga acaactttac
      661 cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag aatagctgat gtagtctcag
      721 tgagcccata tccttgtcgt atccctggaa gatggaagcg ttttgcaacc gcttccccga
      781 cttctttcga aagaggtgcg cccccagaag caatttcgtg taaattagat aaatcgtatt
      841 tgtcaatcag agtgc
//
