LOCUS       sequence-93719-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses in Escherichia coli Notexin with a N-terminal extra 
            methionine and with the amino acid substitution N1S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Simonato M, Morbiato L, Zorzi V, Caccin P, Fernandez J, Massimino 
            ML, Polverino de Laureto P, Tonello F
  TITLE     Production in Escherichia coli, folding, purification and 
            characterization of notexin with wild type sequence and with 
            N-terminal and catalytic site mutations.
  JOURNAL   Toxicon. 2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 
            10.1016/j.toxicon.2014.06.009.
  PUBMED    24951874
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicon. 
            2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 
            10.1016/j.toxicon.2014.06.009."
COMMENT     Sequence Label: pET-20b-M-Ntx-S1
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             450..472
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(503..522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(504..522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccc tactggcaac gttttttggt gtcgatgttc cagttagcgt
      121 tgttgtacgg agctttagcg aagcagaaag cagcttcaac gtcgcagtcg caaacgaaac
      181 gcaggcattt ttttttgatg ttacggcagt acggaccgtt ttcaccgcag tagtagtcgt
      241 aagcagacat tttcgggaag caaccttttt taccagcttc gtcgtagcag tcgtcgtgga
      301 ttttgcagca acggtccagt tcgtcaaccg gggtaccaga accaccagca ccgcagtagc
      361 aaccgtagtc catgtagtgc caggtcggac gtttaccgtg gttagcgcac tggatcaggt
      421 aagagaactg aaccaggctc atatgtatat ctccttctta aagttaaaca aaattatttc
      481 tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg ggatcgagat
      541 ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
      601 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
      661 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
      721 cggtttccgt gtttcgtaaa gtctgg
//
