LOCUS       sequence-93725-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucC-hDib1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit-CO-hDib1
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(490..513)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      513..522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             528..554
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgatcactcg aggtagcggt acttggtgga
       61 gtagtccttg ggggacacca ccaggccgcg gcctttgcgg gccccgcggt acaccgtctc
      121 gatgatgtcc accatctcct gcttgtcctc catggcccag ttaatcttgt tgttgttgcc
      181 agtccccaag tcaatcatga tgtgcttgtt cctgaagaaa aacatgacag tacatggatc
      241 gtataactca tacattttgt tgaagtcagg cacttctgta atatccacaa gataaataac
      301 tgcaaaattt ttaaccttct cggcgatgct gtacaggacc tcgtccatct tcatgcacgt
      361 aggatcccag tcgtggccga agcggatgac gaccacgcgg tcctcctccg agaggatggc
      421 ctggtccacc tgccagccgt tgtgcaggtg cgggagcatg tacgacatga attcaactag
      481 agctagttcc ttgtcatcgt cgtccttgta gtccatggtg gcggatccac caccaccact
      541 accaccacca ccaccggtac ccaatttgga ctttccgccc ttcttggcct ttatgaggat
      601 ctctctgatt tttcttgcgt cgagttttcc ggtaagacct ttcggtactt cgtccacaaa
      661 cacaactcct ccgcgcaact ttttcgcggt tgttacttga ctggcgacgt aatccacgat
      721 ctctttttcc gtcatcgtct ttccgtgctc caaaacaaca acggcggcgg gaagttcacc
      781 ggcgtcatcg tcgggaagac ctgccacgcc cgcgtcgaag atgttggggt gttgtaacaa
      841 tatcgattcc aattcagcgg gggccacctg atatcctttg tatttaatta aagacttcaa
      901 gcggtcaact atg
//
