LOCUS       sequence-93727-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tag protein of interest with PIF6 in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Yang X, Jost AP, Weiner OD, Tang C
  TITLE     A light-inducible organelle-targeting system for dynamically 
            activating and inactivating signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 
            10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.
  PUBMED    23761071
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0126"; journalName: "Mol Biol Cell"; date: 
            "2013-08"; volume: "24"; issue: "15"; pages: "2419-30"
COMMENT     Sequence Label: linker-mCitrine-PIF6-NAT
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..726
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 attaacaaag caatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
       61 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
      121 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
      181 tggccaacct tagtcactac tttaggttat ggtttgatgt gttttgctag atacccagat
      241 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
      301 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
      361 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt
      421 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa
      481 caaaagaatg gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt
      541 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
      601 gacaaccatt acttatccta tcaatctaga ttatccaaag atccaaacga aaagagagac
      661 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
      721 tacaaaggta gttccctagg ttcagggagc ggatccggct cagggagtag cgatatgatg
      781 ttcttaccaa ccgattattg ttgca
//
