LOCUS       sequence-93728-e        1244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Nomme J, Fanning AS, Caffrey M, Lye MF, Anderson JM, Lavie A
  TITLE     The Src homology 3 domain is required for junctional adhesion 
            molecule binding to the third PDZ domain of the scaffolding protein 
            ZO-1.
  JOURNAL   J Biol Chem. 2011 Dec 16;286(50):43352-60. doi: 
            10.1074/jbc.M111.304089. Epub 2011 Oct 26.
  PUBMED    22030391
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.304089"; journalName: "J Biol Chem"; date: 
            "2011-12-16- 16"; volume: "286"; issue: "50"; pages: "43352-60"
COMMENT     Sequence Label: pET14b_tev_PSG
FEATURES             Location/Qualifiers
     source          1..1244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     RBS             524..532
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ccatgggcag cagccatcat catcatcatc acagcagcgg cggtaacgag aatctttatt
       61 ttcagggcca tatgattctt cggcccagca tgaaattggt aaaattcaga aaaggagata
      121 gtgtgggttt gcggctggct ggtggaaatg atgttggaat atttgtagct ggcgttctag
      181 aagatagccc tgcagccaag gaaggcttag aggaaggtga tcaaattctc agggtaaaca
      241 acgtagattt tacaaatatc ataagagaag aagccgtcct tttcctgctt gacctcccta
      301 aaggagaaga agtgaccata ttggctcaga agaagaagga tgtttatcgt cgcattgtag
      361 aatcagatgt aggagattct ttctatatta gaacccattt tgaatatgaa aaggaatctc
      421 cctatggact tagttttaac aaaggagagg tgttccgtgt tgtggatacc ttgtacaatg
      481 gaaaactggg ctcttggctt gctattcgaa ttggtaaaaa tcataaggag gtagaacgag
      541 gcatcatccc taataagaac agagctgagc agctagccag tgtacagtat acacttccaa
      601 aaacagcagg cggagaccgt gctgacttct ggagattcag aggtcttcgc agctccaaga
      661 gaaatcttcg aaaaagcaga gaggatttgt ccgctcagcc tgttcaaaca aagtttccag
      721 cttatgaaag agtggttctt cgagaagctg gatttctgag gcctgtaacc atttttggac
      781 caatagctga tgttgccaga gaaaagctgg caagagaaga accagatatt tatcaaattg
      841 caaagagtga accacgagac gctggaactg accaacgtag ctctggcatt attcgcctgc
      901 atacaataaa gcaaatcata gatcaagaca agcatgcatt attagatgta acaccaaatg
      961 cagttgatcg tcttaactat gcccagtggt atccaattgt tgtatttctt aaccctgatt
     1021 ctaagcaagg agtaaaaaca atgagaatga ggttatgtcc agaatctcgg aaaagtgcca
     1081 ggaagttata cgagcgatct cataaacttc gtaaaaataa tcaccatctt tttacaacta
     1141 caattaactt aaattcaatg aatgatggtt ggtatggtgc gctgaaagaa gcaattcaac
     1201 aacagcaaaa ccagctggta tgggtttccg agggatgagg atcc
//
