LOCUS       sequence-93732-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LucC alone in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pcDNA3-FLAG-Luc-C
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(536..559)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(575..594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(576..594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(639..842)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(668..688)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagttacaa tttggacttt ccgcccttct
       61 tggcctttat gaggatctct ctgatttttc ttgcgtcgag ttttccggta agacctttcg
      121 gtacttcgtc cacaaacaca actcctccgc gcaacttttt cgcggttgtt acttgactgg
      181 cgacgtaatc cacgatctct ttttccgtca tcgtctttcc gtgctccaaa acaacaacgg
      241 cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc cacgcccgcg tcgaagatgt
      301 tggggtgttg taacaatatc gattccaatt cagcgggggc cacctgatat cctttgtatt
      361 taattaaaga cttcaagcgg tcaactatga agaagtgttc gtcttcgtcc cagtaagcta
      421 tgtctccaga atgtagccat ccatccttgt caatcaaggc gttggtcgct tccggattgt
      481 ttacataacc ggacataatc ataggtccgg atccgaattc aactagagct agttccttgt
      541 catcgtcgtc cttgtagtcc ataagcttgg gtctccctat agtgagtcgt attaatttcg
      601 ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc
      661 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
      721 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
      781 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
      841 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
      901 gtcatgtact gggcataatg ccaggcgggc atttaccgtc attgacgtca ata
//
