LOCUS       sequence-93753-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-nEF Con/Fon hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    156..189
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     regulatory      196..205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(641..674)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(725..758)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 agaataccag tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg
       61 atatcgaatt cgaagttcct attcttcaaa aggtatagga acttctcttt gcaccattct
      121 aaagaataac agtgataatt tctgggttaa ggcaagaagt tcctattctt caaatagtat
      181 aggaacttct ctagagccac catggactat ggcggcgctt tgtctgccgt cggacgcgaa
      241 cttttgttcg ttactaatcc tgtggtggtg aacgggtccg tcctggtccc tgaggatcaa
      301 tgttactgtg ccggatggat tgaatctcgc ggcacgaacg gcgctcagac cgcgtcaaat
      361 gtcctgcagt ggcttgcagc aggattcagc attttgctgc tgatgttcta tgcctaccaa
      421 acctggaaat ctacatgcgg ctgggaggag atctatgtgt gcgccattga aatggttaag
      481 gtgattctcg agttcttttt tgagtttaag aatccctcta tgctctacct tgccacagga
      541 caccgggtgc agtggctgcg ctatgcagag tggctgctca cttgtcctgt catccttatc
      601 cgcctgagca acctcaccgg taagtgtcgg ggttgctagc ataacttcgt atagtatacc
      661 ttatacgaag ttattctttg caccattcta aagaataaca gtgataattt ctgggttaag
      721 gcaaataact tcgtatagga tactttatac gaagttatgg tacccctcct gtactcacct
//
