LOCUS       sequence-93757-e         473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-hSyn Con/Fon hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..191
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     regulatory      198..207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
       61 tgatatcgaa ttcgaagttc ctattcttca aaaggtatag gaacttctct ttgcaccatt
      121 ctaaagaata acagtgataa tttctgggtt aaggcaagaa gttcctattc ttcaaatagt
      181 ataggaactt cgagctcgcc accatggact atggcggcgc tttgtctgcc gtcggacgcg
      241 aacttttgtt cgttactaat cctgtggtgg tgaacgggtc cgtcctggtc cctgaggatc
      301 aatgttactg tgccggatgg attgaatctc gcggcacgaa cggcgctcag accgcgtcaa
      361 atgtcctgca gtggcttgca gcaggattca gcattttgct gctgatgttc tatgcctacc
      421 aaacctggaa atctacatgc ggctgggagg agatctatgt gtgcgccatt gaa
//
