LOCUS       sequence-93762-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-nEF Con/Foff hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(190..223)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(274..307)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
ORIGIN
        1 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
       61 agcactgcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg ggcagctgtg
      121 ggacgacatg acttaaccag ttaaccatgt tctctgaaca tgagaagggc tggccactct
      181 ccagtcgaca taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
      241 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagtat accttatacg
      301 aagttatact agttgtgccc cccctttttt ttataaattg tattaatgtt atatacatat
      361 ctcctgtatg tgacccatgt gcttatgact ctatttctca tgtgtttagg cctgagcaac
      421 gactacagca ggagaaccat gggactcctt gtctcagaca tcgggactat cgtgtggggg
      481 gctaccagcg ccatggcaac cggctatgtt aaagtcatct tcttttgtct tggattgtgc
      541 tatggcgcga acacattttt tcacgccgcc aaagcatata tcgagggtta tcatactgtg
      601 ccaaagggtc ggtgccgcca ggtcgtgacc ggcatggcat ggctgttttt cg
//
