LOCUS       sequence-93771-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    155..188
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(640..673)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(724..757)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg ataagcttga
       61 tatcgaattc gaagttccta ttcttcaaaa ggtataggaa cttctctttg caccattcta
      121 aagaataaca gtgataattt ctgggttaag gcaagaagtt cctattcttc aaatagtata
      181 ggaacttcga gctcgccacc atggactatg gcggcgcttt gtctgccgtc ggacgcgaac
      241 ttttgttcgt tactaatcct gtggtggtga acgggtccgt cctggtccct gaggatcaat
      301 gttactgtgc cggatggatt gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg
      361 tcctgcagtg gcttgcagca ggattcagca ttttgctgct gatgttctat gcctaccaaa
      421 cctggaaatc tacatgcggc tgggaggaga tctatgtgtg cgccattgaa atggttaagg
      481 tgattctcga gttctttttt gagtttaaga atccctctat gctctacctt gccacaggac
      541 accgggtgca gtggctgcgc tatgcagagt ggctgctcac ttgtcctgtc atccttatcc
      601 gcctgagcaa cctcaccggt aagtgtcggg gttgctagca taacttcgta tagtatacct
      661 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      721 caaataactt cgtataggat actttatacg aagttatggt acctgtgccc cccctttttt
      781 ttataaattg tattaatgtt atatacatat ctcctgtatg tgacccatgt gcttatgact
      841 ctatttctca tgtgtttag
//
