LOCUS       sequence-93772-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-on EYFP under the Synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-hSyn Con/Fon EYFP
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..136)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(143..176)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(227..260)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      265..274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(316..337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    450..483
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    534..567
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gtacaagtaa ggcgcgccga agttcctata ccttttgaag aataggaact tcctgggcag
       61 tataaagact taaggaagtg attctttgca ccaataacaa ttgaagttcc tatactattt
      121 gaagaatagg aacttcggta ccataacttc gtatagtata ccttatacga agttattctt
      181 tgcacctatc taaagaataa cagtgataat ttctgggtta aggcaaataa cttcgtatag
      241 gatactttat acgaagttat gctagccacc atggtgagca agggcgagga gctgttcacc
      301 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      361 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      421 accggcaagg tgagtacagg aggactagta taacttcgta taaggtatac tatacgaagt
      481 tattaagcgc agctagaggt aatacaacaa tcttttcaaa ttgctccagc tacataactt
      541 cgtataaagt atcctatacg aagttatgga tccttctcga ctgcgctctc aggcacgaca
      601 cgactcctcc gctgcccacc gcagactgag gcagcgctga gtcgccggcg ccgca
//
