LOCUS       sequence-93776-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-on/Flp-off EYFP under the Synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2996"; journalName: "Nat Methods"; date: "2014-07"; 
            volume: "11"; issue: "7"; pages: "763-72"
COMMENT     Sequence Label: pAAV-hSyn Con/Foff EYFP
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(277..310)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(317..350)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(401..434)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      439..448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(490..511)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
       61 aagcactgca ggccgtagcc gaaggtggtc acgagggtgg gccagggcac gggcagctgt
      121 gggacgacat gacttaacca gttaaccatg ttctctgaac atgagaaggg ctggccactc
      181 tccaggcgcg ccgaagttcc tatacctttt gaagaatagg aacttcctgg gcagtataaa
      241 gacttaagga agtgattctt tgcaccaata acaattgaag ttcctatact atttgaagaa
      301 taggaacttc ggtaccataa cttcgtatag tataccttat acgaagttat tctttgcacc
      361 tatctaaaga ataacagtga taatttctgg gttaaggcaa ataacttcgt ataggatact
      421 ttatacgaag ttatgctagc caccatggtg agcaagggcg aggagctgtt caccggggtg
      481 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      541 gagggcgagg gcgatgccac ctacggcaag ctgaccctga
//
