LOCUS       sequence-93779-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-Dependent Chloride Channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Berndt A, Lee SY, Ramakrishnan C, Deisseroth K
  TITLE     Structure-guided transformation of channelrhodopsin into a 
            light-activated chloride channel.
  JOURNAL   Science. 2014 Apr 25;344(6182):420-4. doi: 10.1126/science.1252367.
  PUBMED    24763591
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-25- 25"; volume: "344"; 
            issue: "6182"; pages: "420-4"
COMMENT     Sequence Label: pAAV-Ef1a-DIO SwiChRca-TS-EYFP
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(70..103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(154..187)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      192..201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat
       61 atcgaattca taacttcgta tagcatacat tatacgaagt tattctttgc accattctaa
      121 agaataacag tgataatttc tgggttaagg caaataactt cgtataggat actttatacg
      181 aagttatgct agccaccatg gactatggcg gcgctttgtc tgccgtcgga ctcttccaaa
      241 cctcatacac tcttgagaac aatggttctg ttatttgcat cccgaacaac ggccagtgct
      301 tctgcttggc ttggcttaaa tccaacggaa caaatgccga gaagttggct gccaacattc
      361 tgcagtggat ttcttttgcg ctttcagcgc tctgcctgat gttctacggc taccagacct
      421 ggaagtctac ttgcggctgg gaggagattt acgtggccac gatctcgatg atcaagttca
      481 tcatcgagta tttccatagc tttgacgaac ctgcggtgat ctactcatcc aacggcaaca
      541 agaccaagtg gcttcgttac gcgagctggc tgctgaccgc ccctgtcatt cttatccgtc
      601 tgagcaacct tacgggtctg gcgaacgact ataacaagcg taccatgggt ctgctggtgt
      661 cagatatcgg cacgatcgtg tggggcacca cggccgcgct gtccaaggga tacgtccgtg
      721 tcattttctt cctgatgggc ctgtgctacg gcatctacac attcttcaac gcagccaag
//
