LOCUS       sequence-9379-ex         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..541
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     460..480
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(668..688)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(720..742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
       61 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
      121 ctcgcagcca acgtcggggc ggcaggccct ttgacgtcaa tgggagtttg ttttggcacc
      181 aaaatcaacg ggacttctcg agccttggct agaactagtg gatccgacgc cgccatctct
      241 aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc
      301 ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca
      361 gataatcaga tctctgttct ttttaatact agctacattt tacatgatag gcttggattt
      421 ctataagaga tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc
      481 ctaactgtaa agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt
      541 ttaaacacca cttcctgtca gttaactttt tgaattcttt gttaggcctg gtacctctag
      601 agatatcctg cagtggccaa tgcattggtt cgcggccgcc accgcggtgg agctccagct
      661 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      721 ctgtgtgaaa ttgttatccg ctcgggccct gccatagcct caggttactc atatatactt
      781 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      841 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      901 gaaaagatca aaggatcttc
//
