LOCUS       sequence-93799-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible HA-tagged mouse 
            Tgfbr3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pSLIK Tgfbr3-HA neo
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        135..449
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    214..232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    286..304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    357..375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     422..446
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
       61 aattctagac agttatcaac aagtttgtac aaaaaagcag gctttaaagg aaccaattca
      121 gtcgactgga tcccgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      181 cctatcagtg atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt
      241 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      301 gagaacgtat gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc
      361 tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca
      421 gagctcgttt agtgaaccgt cagatcgcca ctagtatggc agtgacatcc caccacatgg
      481 tccctgtgtt tgtcctgatg agcgcctgcc tggccaccgc aggtccagag cccagcaccc
      541 ggtgtgaact gtcaccgatc agtgcctctc atccagtcca ggccctgatg gagagcttca
      601 ccgttctgtc tggctgtgcc agcagaggca ccactgggct gccaagggag gttcacatcc
      661 taaacctccg cagtacagac caaggactag gccagccgca gagagaggtt accctgcatc
      721 tgaaccccat tgcctccgtg cacactcacc acaagcctgt tgtgttcctg ctcaactccc
      781 cacagcccct ggtgtggc
//
