LOCUS       sequence-93811-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible HA-tagged rat 
            S6K1-E389-deltaCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pSLIK S6K (E389-deltaCT) neo
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        139..453
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    147..165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    254..272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    290..308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    325..343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    361..379
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     426..450
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             463..489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
       61 ggttaattct agacagttat caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa
      121 ttcagtcgac tggatcccga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      181 actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt
      241 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      301 gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac
      361 tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata
      421 agcagagctc gtttagtgaa ccgtcagatc gccactagaa tgtacccata cgatgttcca
      481 gattacgctg caggagtgtt tgacatagac ctggaccagc cagaggatgc aggctctgag
      541 gatgagctgg aggagggggg tcagttaaat gaaagcatgg accatggggg agttggacca
      601 tatgaacttg gcatggaaca ttgtgagaaa tttgaaatct cagaaactag tgtgaacaga
      661 gggccagaaa aaatcagacc agaatgtttt gagctacttc gggtacttgg taaagggggc
      721 tatggaaaag gtttttcaag tacgaaaagt aacaggagca aatactggga agatatttgc
      781 catgaaggtg cttaaaaagg caatga
//
