LOCUS       sequence-93816-e         542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for knockdown of human TGFBR3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pLKO.1 shTGFBR3 puro
FEATURES             Location/Qualifiers
     source          1..542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(54..302)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(112..131)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(282..302)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aaaccaagca tgaaggaacc aaatctcgag atttggttcc ttcatgcttg gccggtgttt
       61 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
      121 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
      181 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt
      241 atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc
      301 tcggtgaagg gggcggccgc tcgaggctag tctcgtgatc gataccgtcg agatccgttc
      361 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
      421 ctgtcgggtc ccctcggggt tgggaggggg gtctgaaacg ataatggtga atatccctgc
      481 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
      541 ac
//
