LOCUS       sequence-93819-e        1485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic excitation of ES cell-derived neurons (and other cells, 
            such as muscle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, 
            Burrone J, Greensmith L, Lieberam I
  TITLE     Optical control of muscle function by transplantation of stem 
            cell-derived motor neurons in mice.
  JOURNAL   Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.
  PUBMED    24700859
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-4- 4"; volume: "344"; issue: 
            "6179"; pages: "94-7"
COMMENT     Sequence Label: tol2-CAG::ChR2-YFP
FEATURES             Location/Qualifiers
     source          1..1485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..62
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        92..196
                     /gene="bla"
                     /label=AmpR promoter
     promoter        198..555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        198..389
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(221..241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      406..541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        603..650
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             669..1043
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1173..1294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1210..1229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1264..1283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1336..1355)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1380..1427
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    1432..1479
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ttaattaacc cggggaagtt cctattccga agttcctatt ctctagaaag tataggaact
       61 tccgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      121 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      181 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
      241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      301 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      361 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      421 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      481 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      541 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
      601 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
      661 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
      721 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
      781 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
      841 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
      901 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
      961 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     1021 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     1081 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     1141 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     1201 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1261 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgaattccc ggggatcctc
     1321 tagggggagg ctgactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     1381 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata agaagttcct
     1441 attccgaagt tcctattctc tagaaagtat aggaacttcg tcgac
//
