LOCUS       sequence-93821-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty lentiviral expression vector for ultradestabilized Venus that 
            indicates the activity of a transcriptional element of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pTRF.1-mCMV-udsVenus
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..410)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(201..222)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     550..569
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac tacacattga tcctagcaga agcacaggct
       61 gcagggtgac ggtccatccc gctctcctgg gcacaagaca tgggcagcgt gccatcatcc
      121 tgcgccgcca ccgccggcgg gaagccatgg ctgatatcct tgtacagctc gtccatgccg
      181 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      241 gggtctttgc tcagggcgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg
      301 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc gccgtcctcg
      361 atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata
      421 tagacgttgt ggctattgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      481 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      541 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      601 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtc
//
