LOCUS       sequence-93824-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A rapidly responsive reporter of endogenous JUND promoter activity 
            (Lentiviral expression vector for reporter ultradestabilized Venus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pTRF.1 udsVenus (P_JUND)
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(63..290)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(362..386)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctccctcgc ccttgctcac catggtggcg accggtggat cctgtcgacc aagcttcccg
       61 cgcccacctc tgagacggag caccaggtgc agggtggact ctttctggat gttgtagtca
      121 gacagggtgc gcccatcttc cagctgtttt ccagcaaaga tcagcctctg ctggtcagga
      181 gggatgcctt ccttatcttg gatctttgcc ttgacattct cgatggtgtc actgggctca
      241 acctcgaggg tgatggtctt accagtcaga gtcttcacga agatctgcat ggtaagctta
      301 ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
      361 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctaa
      421 ctagtctcca ctggcggcgg ctcctgcgcc gcgccacttg tgcgccctct tatagcctcg
      481 gctcggcgcg atgagctcac cgcccccgtt cgccattggg tgcgggtgac gtcgctcatt
      541 tgcatggagg ggcggggcca agccgccgac gcccgcccgg ccgccgttgc cccggcgacg
      601 cgcggtaaac gccacaacag cgcgctgcct tgtgggttga cgtcatgggg gcggccgcgc
      661 gcccgggccg ggtcaccccg ccccgggacc gctgtgtacg accgatctcc c
//
