LOCUS       sequence-93825-e         465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A rapidly responsive reporter of endogenous JUND promoter activity 
            (Lentiviral expression vector for reporter ultradestabilized Venus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pTRF.1 udsVenus (P_JUND)
FEATURES             Location/Qualifiers
     source          1..465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(161..412)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(203..224)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caaattttgt aatccagagg ttgattgtcg actacacatt gatcctagca gaagcacagg
       61 ctgcagggtg acggtccatc ccgctctcct gggcacaaga catgggcagc gtgccatcat
      121 cctgcgccgc caccgccggc gggaagccat ggctgatatc cttgtacagc tcgtccatgc
      181 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      241 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
      301 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct
      361 cgatgttgtg gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga
      421 tatagacgtt gtggctattg tagttgtact ccagcttgtg cccca
//
