LOCUS       sequence-93827-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Taura T, Krebber H, Silver PA
  TITLE     A member of the Ran-binding protein family, Yrb2p, is involved in 
            nuclear protein export.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Jun 23;95(13):7427-32.
  PUBMED    9636166
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1998-06-23"; volume: "95(13)"; pages: 
            "7427-32"
COMMENT     Sequence Label: pPS1372
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             155..175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             269..985
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(302..330)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     925..945
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
       61 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      121 accaagcata caataagcat cgatgggtgc tcctccaaaa aagaagagaa aggtagctgg
      181 tatcaataaa gacatccccg ggttagcctt gaaattagca ggtcttgata tcaacaagac
      241 aggaatcgac aaaaatatcg gtgaattcat ggctagcaaa ggagaagaac tcttcactgg
      301 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      361 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      421 tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg gtgttcagtg
      481 cttttcaaga tacccggatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      541 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      601 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      661 taaagaagat ggaaacattc ttgggcacaa attggaatac aactataact cacacaatgt
      721 atacatcatg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttagacacaa
      781 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      841 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      901 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      961 acatggcatg gatgaactat acaaagctcg agctatggct agcaaaggag aagaactctt
     1021 cactggagtt gtc
//
