LOCUS       sequence-93828-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses eGFP from a 400 bp human FOXA2 promoter to select floor 
            plate and ventral midbrain progenitor cells during development .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Aguila JC, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, 
            Gayosso M, Paz Lopez Mato M, Lopez de Maturana R, Hedlund E, Sonntag
            K, Sanchez Pernaute R
  TITLE     Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for 
            Dopamine Neurons From Human Pluripotent Stem Cells.
  JOURNAL   Stem Cells Transl Med. 2014 Jul 14. pii: sctm.2014-0011.
  PUBMED    25024431
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 Jul 14. pii: sctm.2014-0011."
COMMENT     Sequence Label: Lenti.pFOXA2.GFP
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    104..221
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      649..658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(700..721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
       61 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
      121 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      181 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc
      241 tcgagcaagg tccagccagg ttttcagaac caagaaagag cctccacatc caaacacctg
      301 caatatcccc ccactccaaa tctgggctca caggctaacc cagaacagaa gacaattttt
      361 gaacccaaga gctgctgggg aaataaaagt atacgattgc tggagtttct aatttctatt
      421 aagcagtccc tctggaagac agagaggaca gagacgctct tgaagtcaac tccatatgcc
      481 ccatcattga ttcctggatt cttctctcct cacccctccc tccccatctc ctgccctgtt
      541 tgttttagtt acgaaatgct gtgggcacct cggttgtgac tgaaaagtaa ccttgaaaca
      601 cgccggcctg aatatcagag gcaaatctca gcctcccaac caccggtcgc caccatggtg
      661 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      721 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      781 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      841 accaccctga cctacggcgt g
//
