LOCUS       sequence-93830-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses eGFP from a 400 bp human FOXA2 promoter to select floor 
            plate and ventral midbrain progenitor cells during development .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Aguila JC, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, 
            Gayosso M, Paz Lopez Mato M, Lopez de Maturana R, Hedlund E, Sonntag
            K, Sanchez Pernaute R
  TITLE     Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for 
            Dopamine Neurons From Human Pluripotent Stem Cells.
  JOURNAL   Stem Cells Transl Med. 2014 Jul 14. pii: sctm.2014-0011.
  PUBMED    25024431
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 Jul 14. pii: sctm.2014-0011."
COMMENT     Sequence Label: Lenti.pFOXA2.GFP
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(54..767,768..770,771..773))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(57..767,768..770,771..773))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(99..120)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     448..467
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     707..728
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacgcggcc gctttacttg
       61 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      121 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      181 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      241 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
      301 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      361 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      421 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      481 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      541 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
      601 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      661 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      721 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg
      781 accggtggtt gggaggctga gatttgcctc tgatattcag gccggcgtgt ttcaaggtta
      841 cttttcagtc acaaccgagg tgcccacagc atttcgtaac taa
//
