LOCUS       sequence-93832-e         386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses eGFP from an about 1350 bp human FOXA2 promoter to select 
            floor plate and ventral midbrain progenitor cells during 
            development.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Aguila JC, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, 
            Gayosso M, Paz Lopez Mato M, Lopez de Maturana R, Hedlund E, Sonntag
            K, Sanchez Pernaute R
  TITLE     Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for 
            Dopamine Neurons From Human Pluripotent Stem Cells.
  JOURNAL   Stem Cells Transl Med. 2014 Jul 14. pii: sctm.2014-0011.
  PUBMED    25024431
REFERENCE   2  (bases 1 to 386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 Jul 14. pii: sctm.2014-0011."
COMMENT     Sequence Label: Lenti.pFOXA2.5'.GFP
FEATURES             Location/Qualifiers
     source          1..386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttgctcac catggtggcg accggtacag ccacaacaaa cgaccagcaa tcacccccca
       61 cccccaccct cttttaaaaa aaagtcagcc aaagcaccgt cccctcctcc ctccctctct
      121 cgcgctccct ctccctgggc tccactccct ctctctccct gggcaggccg gaggcggtag
      181 ttggaagtgg gcgggaggtg ggggggggcg aggagcggag gaggcccagg ccagcgcccc
      241 gcggtaggga gcacccgccg ccgccgcgct cacgggctgc cgggtggcgg ctgaggttgg
      301 cagtgccgag ctgccccgag gcggcgggaa gcgcgcggcg cgggggctag tgggggggtg
      361 gggaggagga ggaggaagga ggagga
//
