LOCUS       sequence-93835-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zeocin-resistance plasmid for insertional mutagenesis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Mukaiyama H, Oku M, Baba M, Samizo T, Hammond AT, Glick BS, Kato N, 
            Sakai Y
  TITLE     Paz2 and 13 other PAZ gene products regulate vacuolar engulfment of 
            peroxisomes during micropexophagy.
  JOURNAL   Genes Cells. 2002 Jan;7(1):75-90.
  PUBMED    11856375
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells."; date: "2002-01"; volume: "7(1)"; pages: "75-90"
COMMENT     Sequence Label: pREMI-Z
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(137..384)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     350..368
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(450..824)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(843..890)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttggtctc cagcttgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg
      181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa
      241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt
      301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta
      361 agcgtgacat aactaattac atgatatcga caaaggaaaa gggggacgga tctccgaggc
      421 ctcggacccg tcgggccgcg tcggacgtgt cagtcctgct cctcggccac gaagtgcacg
      481 cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg
      541 gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg
      601 tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg
      661 tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc
      721 tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg
      781 tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc
      841 ttgtcgtatt atactatgcc gatatactat gccgatgatt aattgtcaac accgccctta
      901 gattagattg ctatgctttc tttctaatga gcaagaagta aaaaa
//
