LOCUS       sequence-93858-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for an inducible HA-tagged mouse Tgfbr3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA
  TITLE     A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in 
            single breast epithelial cells and basal-like premalignancies.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 
            2014 Mar 23.
  PUBMED    24658685
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2930"; 
            journalName: "Nat Cell Biol"; date: "2014-04"; volume: "16"; issue: 
            "4"; pages: "345-56"
COMMENT     Sequence Label: pEN_TT Tgfbr3-HA
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..121
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        153..467
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    268..286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    304..322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    339..357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    375..393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     440..464
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tagttactta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
       61 ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc
      121 tttaaaggaa ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa
      181 cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca
      241 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      301 tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg
      361 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
      421 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccact agtatggcag
      481 tgacatccca ccacatggtc cctgtgtttg tcctgatgag cgcctgcctg gccaccgcag
      541 gtccagagcc cagcacccgg tgtgaactgt caccgatcag tgcctctcat ccagtccagg
      601 ccctgatgga gagcttcacc gttctgtctg gctgtgccag cagaggcacc actgggctgc
      661 caagggaggt tcacatccta aacctccgca gtacagacca aggactaggc cagccgcaga
      721 gagaggttac cctgcatctg aaccccattg cctccgtgca cactcaccac aagcctgttg
      781 tgttcctgct caactcccca cagcccctgg tgtggcatgt gaagacagag agactggctg
      841 ctggtgtccc cagactcttc ctggtttcgg a
//
