LOCUS       sequence-93873-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HTLV-1 transfer vector that encodes firefly luciferase gene 
            interrupted by a gamma-globin intron. The reporter gene is 
            transduced only after the completion of viral cycle replication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Mazurov D, Ilinskaya A, Heidecker G, Lloyd P, Derse D
  TITLE     Quantitative comparison of HTLV-1 and HIV-1 cell-to-cell infection 
            with new replication dependent vectors.
  JOURNAL   PLoS Pathog. 2010 Feb 26;6(2):e1000788. doi: 
            10.1371/journal.ppat.1000788.
  PUBMED    20195464
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2010-02-26"; volume: "6(2):e1000788. doi"; pages: "
            10.1371/journal.ppat.1000788"
COMMENT     Sequence Label: pCRU5HT1-inLuc
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..78
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(93..98,99..116,117..123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    131..147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     136..158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     155..171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     155..171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        269..648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
       61 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      181 gccaagcttg atatcccatt gcatacgttg tatccatatc ataatatgta catttatatt
      241 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
      301 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      361 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      421 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      481 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      541 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      601 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      661 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      721 cccattgacg tcaat
//
