LOCUS       sequence-9392-ex       10206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: psiCHECK RBJ 3'UTR
FEATURES             Location/Qualifiers
     source          1..10206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     604..623
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    5621..5669
                     /note="synthetic polyadenylation signal"
     promoter        5678..6429
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      6459..6468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6465..8117
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             7424..7432
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8161..8282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8172..8191)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8226..8245
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8415..8519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8520..8588,8589..9380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8738..8757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9551..10139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10040..10059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgaggc tcggacagcc ctcctgaaaa acatcaagta
     1681 gaaagtacag aaaaaagcca catgtgggac tcaaatgcaa acagactttc cctagaggtg
     1741 aaataaccaa cgtggagttt tccttcccag aatctcactg ctcttttcat tcatgtgttg
     1801 tcatttgtat atcagtaatt caggtaccca tttcatagac attttactga gaaatgacct
     1861 gcatttgtat gaagtgaact gagcgtcaca ccctgtactt catttcatat ttctagataa
     1921 ttctgaattt ttttctcatt cgtcagctct gtaattatag tatcacttag acatttcact
     1981 tggggaaatc cacaaggttc ctggaggagg gaagagagga caagaggacc ctttcacttt
     2041 ttctttttta cggaattcat catcagagaa gaaaataaca aaaatggaag caaacaacat
     2101 cagaacccct gtaagtttgg tgtgacctta cagacaagtt gctgctttta caatgagttc
     2161 cttaggtggt attttaaccc atcgatctat aatgatgact cttggcagcc ctttgggagt
     2221 ttgtaaaatg aggtgataca gttctgaatt gagcattcct ttatgatatt cactctgttc
     2281 ctcttctgca gccaccagtg ggagagacaa gccagtccta agagaaaagg tggtggcagc
     2341 cacaaattct aggtacactg gctgctgcct atcctgtccc tggatctgag gcctttccct
     2401 tgccatagaa atggttgctg gtagcagtag agagcactgt gcacctggga atgaggaatc
     2461 aggccccaag acagaagtac ttggaggagc cagctgcagt agtatccgcc tgtagtccca
     2521 gctactcagg aggctgagac aggaggattg cttaagccca ggagctcaag tcccacctgg
     2581 gcaacatagt aagatcttgt ctcttaaaga aaaaaaaaaa aggtacttag aggtcgcact
     2641 taaagattat gcacatcagc agaggaaagg ccagccaagc ttggggcaag cttgatgcag
     2701 taggagagac tccttatgag gcttagccct tgtcttactg ccagcctttg ccacaggcag
     2761 gtgagaaagg cagggccact cctcagcaaa gttggcctct tctacacttc ctcaccagtg
     2821 ttgtttcttc cttttttccc ctcttcccct tcccgtcttg tgttttttct gaaccccaca
     2881 tctgccatca tttctctcct ctagagtccc tggcttctgg ccactgcctc ctccctcttc
     2941 taagccttgg cctgaatctg gttgatcaga ggcaagtgtg gatcctttgg gtggcagtca
     3001 ggggagatct cagggcctct gttgggagga atctctgtaa ttcctgcttg ggctccaaat
     3061 ttctgaagag taatattttt aaactatagc ttacaaaata cattctctga ccacagtctc
     3121 ctccttgata tacaagggat ggatgaagtt catgtattag gactggcact ccttacggtg
     3181 cttatagaac tagtctcaca acttgactca ttacgtcgtc attcttgtta catcactcat
     3241 acttttagct gcaaccacac taattcacat ttttatatac tttcaattag ctgtactaat
     3301 tggggcttga aagtatataa aatcttcctg tcctgtgaat tttaaaaagc tatcccatat
     3361 cgattgccaa agagcatcta ccttacctcc taagaagaaa agccactttc ttccaatcca
     3421 agcccactgc agccttgtgg attttccaca cagcagcttt tcactgtatg cctgtacttg
     3481 ggctgcactg agcttgtctt caggaaacca gagcgttgct ttatcatcgc acttttcatc
     3541 ttggtaatat caaaacaact ttttaaatga actgattgat atatgttatt tcttgcgagg
     3601 ttttcctctg gcctttaact ggcttctgag gctacagaac tccaacccag agttcttcgg
     3661 gacctaaatt ttgcttaagg aaggccttta tcatgctgaa agcactcaga catgtctgtc
     3721 ctcacagcag aatgtagaag tcatatgaat gagggatcgt gcacggtagc gtcagcccga
     3781 attgacacga caactctgaa tgtgtggccc tctatgaaag ctggtgccag ttttcatcac
     3841 tgttattaaa tactagattg ggaacctcat tcagccgtat tgcacagatg agcaattaca
     3901 gcagagaaaa ttcattagtg ctcccactca tatccttatg tgtgagttgg tgaaatttag
     3961 cctgagcttg attcttaggt cttgagactc agtttccaac accttcatta ttaatgttaa
     4021 gaatggccat ataccacttc atttacgtaa aaggaataaa ataagcaagt taagcttcag
     4081 ggtcctagaa tttttctgat ttctataatc cttcagcaga atattaagaa ataaagcatg
     4141 atctgcaatg gaaaggtttt tttggcacta acttcatgca gaatttcaca gacatctatg
     4201 cattaattta aattcgtggt gcaaatgtgt gctttgtagt tcaaccttat taaaaattct
     4261 tcagccagtt aggatttcaa aatacattag tgagagtaac agctagcaga ataattacaa
     4321 tggctaaaac gtttttatag agaatcttgt ttttcaaaaa agaataaaaa gtttgaatag
     4381 ctattattaa agaaaagcca tgtcttcaac atctgagtca ccaagaagta acacttttta
     4441 aacttgttag aaatccctgg aggcatttca aattcatctt ctaacctacc taagcacttt
     4501 aaaatacagc ctccttctgt tcctctgttc cccttcatgc ctccttttga attgatatct
     4561 cagctgtcaa tatttctttg acttcagcat tgttatttta tttctctata tcaatgtgtc
     4621 cttagttttc ttagcctgtt aatttaaaaa tccagacttg ggggtcattg tgttgctacc
     4681 caagagaaaa tgctacagtt ttatagattg cacatcattc tttaaggcat acttttacct
     4741 caggaaactt actctagtat gttctcatat attgaattct caactaagta actatttctt
     4801 tatacctata aactagtacc taattcactg ttagttaaat gagctataga taaggcagct
     4861 aaatttctga aagatagcca gagtaagtaa gcagccactg caagctctga atcagttttt
     4921 taaaaaaaag aaaaaaaaaa aaaaggagct gtgccccatt gaacaatgac aacatcacaa
     4981 ggggaaggga gggattttgc caaaagttag aaaggtggag ctgtaagcca tgtgcttgtt
     5041 gcctttgaca gtgacagtgc gcgtcttaca actaattcca ctaggcatgg gaatgtcacc
     5101 tcctttgcca ctgtacccca tcaagcttac tctgaatcaa aggttttgta aaacttttga
     5161 aaatgaactt agctgcaaaa tatagctaac cactttcaat tgcaagcttt gcaaagtgaa
     5221 gagaggttcg tacttctcat aattggtatt tagcacaatt gtaagataca gttttactgt
     5281 ttgtacatcg tttttttcca ttaaactttt ctagagaaga caaaagctta cactggtgac
     5341 atggataagt gagatggtgt ctaacgagca gtgcaatatt aggcggtgca atagaggccc
     5401 tgttggcaag atgagttttg ctggagagct ggacagtgtt ggttagcttc ctgcattgat
     5461 tgcccctggg cgttcttccc ccttaactgt gaaagcagag tctataaatg aactctttgc
     5521 tcttacaagg taatttacat attttctttc tgttgaaata actgaaaatg ttaaatctac
     5581 cagtttatgt cttgtaaaca gagtggcggc cgctggccgc aataaaatat ctttattttc
     5641 attacatctg tgtgttggtt ttttgtgtga ggatctaaat gagtcttcgg acctcgcggg
     5701 ggccgcttaa gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca
     5761 ctctcattcg gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc
     5821 cgcgatcctc ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg
     5881 taactggcgg gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct
     5941 gctcgcagac ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc
     6001 catggggacc gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc
     6061 ggagaccttc tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac
     6121 aaacaccagg acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag
     6181 gagcttcagg gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc
     6241 ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc
     6301 cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag
     6361 gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga
     6421 cccgcttaaa agcttggcat tccggtactg ttggtaaagc caccatggcc gatgctaaga
     6481 acattaagaa gggccctgct cccttctacc ctctggagga tggcaccgct ggcgagcagc
     6541 tgcacaaggc catgaagagg tatgccctgg tgcctggcac cattgccttc accgatgccc
     6601 acattgaggt ggacatcacc tatgccgagt acttcgagat gtctgtgcgc ctggccgagg
     6661 ccatgaagag gtacggcctg aacaccaacc accgcatcgt ggtgtgctct gagaactctc
     6721 tgcagttctt catgccagtg ctgggcgccc tgttcatcgg agtggccgtg gcccctgcta
     6781 acgacattta caacgagcgc gagctgctga acagcatggg catttctcag cctaccgtgg
     6841 tgttcgtgtc taagaagggc ctgcagaaga tcctgaacgt gcagaagaag ctgcctatca
     6901 tccagaagat catcatcatg gactctaaga ccgactacca gggcttccag agcatgtaca
     6961 cattcgtgac atctcatctg cctcctggct tcaacgagta cgacttcgtg ccagagtctt
     7021 tcgacaggga caaaaccatt gccctgatca tgaacagctc tgggtctacc ggcctgccta
     7081 agggcgtggc cctgcctcat cgcaccgcct gtgtgcgctt ctctcacgcc cgcgacccta
     7141 ttttcggcaa ccagatcatc cccgacaccg ctattctgag cgtggtgcca ttccaccacg
     7201 gcttcggcat gttcaccacc ctgggctacc tgatttgcgg ctttcgggtg gtgctgatgt
     7261 accgcttcga ggaggagctg ttcctgcgca gcctgcaaga ctacaaaatt cagtctgccc
     7321 tgctggtgcc aaccctgttc agcttcttcg ctaagagcac cctgatcgac aagtacgacc
     7381 tgtctaacct gcacgagatt gcctctggcg gcgccccact gtctaaggag gtgggcgaag
     7441 ccgtggccaa gcgctttcat ctgccaggca tccgccaggg ctacggcctg accgagacaa
     7501 ccagcgccat tctgattacc ccagagggcg acgacaagcc tggcgccgtg ggcaaggtgg
     7561 tgccattctt cgaggccaag gtggtggacc tggacaccgg caagaccctg ggagtgaacc
     7621 agcgcggcga gctgtgtgtg cgcggcccta tgattatgtc cggctacgtg aataaccctg
     7681 aggccacaaa cgccctgatc gacaaggacg gctggctgca ctctggcgac attgcctact
     7741 gggacgagga cgagcacttc ttcatcgtgg accgcctgaa gtctctgatc aagtacaagg
     7801 gctaccaggt ggccccagcc gagctggagt ctatcctgct gcagcaccct aacattttcg
     7861 acgccggagt ggccggcctg cccgacgacg atgccggcga gctgcctgcc gccgtcgtcg
     7921 tgctggaaca cggcaagacc atgaccgaga aggagatcgt ggactatgtg gccagccagg
     7981 tgacaaccgc caagaagctg cgcggcggag tggtgttcgt ggacgaggtg cccaagggcc
     8041 tgaccggcaa gctggacgcc cgcaagatcc gcgagatcct gatcaaggct aagaaaggcg
     8101 gcaagatcgc cgtgtaataa ttctagagtc ggggcggccg gccgcttcga gcagacatga
     8161 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     8221 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     8281 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     8341 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt
     8401 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8461 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8521 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8581 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8641 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8701 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8761 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8821 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8881 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8941 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9001 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9061 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9241 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9301 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9361 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9661 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg
    10201 ctcgac
//
