LOCUS       sequence-93935-e         372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  silence doxycyclin inducible pUltra-dox (also expresses luciferase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 372)
  TITLE     pUltra plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Smurf-luc-RTTA3
FEATURES             Location/Qualifiers
     source          1..372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..99)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgccgggcct ttctttatgt ttttggcgtc ttccatacta gaaggcccgg gattctcctc
       61 cacgtcacct gcttgtttga gtagtgagaa gtttgttgct ccagatccct tgtacacgaa
      121 gggcaccacg gaggtgatgt gggccacggc gtgctcggtc agctgcacgc tgttgccgcc
      181 cttgtccagg tcggttctgg cgatgcggtg ctccaccacg tggttggggg gcatggtcac
      241 gggcttcttg gtcttgtagg aggtgtggaa ctggcatctg tagttgccgc cgccctgcag
      301 catcaggaag gcggtcacgt cgcccttcaa gatgccgtcg cacacggtca tcttctcgaa
      361 ggaggggtcc ca
//
