LOCUS       sequence-93936-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pFasBac+GFP-CAMSAP3 CC+CKK
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..33)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(55..72)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(139..230)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(197..214)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(582..587,588..604,605..610)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tggatccatg gcgccctgaa aatacaggtt ttcggtcgtt gggatatcgt aatcgtgatg
       61 gtgatggtga tggtagtacg acatggtttc ggaccgagat ccgcgcccga tggtgggacg
      121 gtatgaataa tccggaatat ttataggttt ttttattaca aaactgttac gaaaacagta
      181 aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat ctattaatat
      241 tccggagtat acgtagccaa ccactagaac tatagctaga gtcctgggcg aacaaacgat
      301 gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca
      361 agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca
      421 ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct
      481 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
      541 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg
      601 ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
      661 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
      721 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
      781 tgttatggag cagcaacgat gttacgcagc agggcagtcg
//
